• Buehler Isomet 1000 Slow Speed Diamond Wafering Saw                 Purpose: Sectioning undemineralized specimens.                 Specifications: Cuts sections to a thickness of 150–250 µm.

• Water–cooled Ecomet 3 grinder/polisher                  Purpose: Finish undemineralized specimens.

Specifications: Variable speed, ultimate specimen thickness 50–100 µm.           • Radial microtome (Scientific Instruments)                  Purpose: Section demineralized or soft tissues.                  Specifications: Produces sections 5–7 µm thick.           • Histomorphometry workstation                  Purpose: Acquire and qualitatively and quantitatively analyze images.                  Specifications: Workstation consists of 1) A Nikon Eclipse E400 microscope with                  1x– 40x objectives, and alternate UV–C light source, 2) in–line color CCD video camera                  (Sony 3ccd), 3) BioQuant histomorphometry software, and 4) SigmaStat and SigmaPlot                  statistics and graphics packages.           • VersaMax Tunable Reader Multiwell Spectrophotometer                  Purpose: measures absorbance or percent transmittance. Quantifies end–point assays                  (ELISAs, cytoproliferation by MTT reduction, colormetric protein assay) and kinetic                  evaluations running up to 99 hours (enzyme activity studies, kinetic ELISAs).                  Specifications: Resolves wavelengths of 340–850 nm.      Additional equipment      This lab also maintains the necessary supplies for performing a variety of histological processing      and staining, including a full paraffin embedding station. Additionally, we have access to a full      tissue culture laboratory facility. MORF also has access to the services provided by the      Minneapolis Medical Research Foundation for qualifying projects. These services include small      animal surgery and housing facilities, and advanced molecular biology capabilities.